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	<title>Protocols &#8211; Anupama Shrestha</title>
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		<title>Receipe for 10*TAE Buffer : 1Litre</title>
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		<dc:creator><![CDATA[Anupama]]></dc:creator>
		<pubDate>Wed, 05 Jan 2011 08:54:50 +0000</pubDate>
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					<description><![CDATA[Preparation of TAE buffer]]></description>
		
		
		
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		<title>CP cell preparation: 100 ml culture</title>
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		<pubDate>Thu, 02 Dec 2010 13:50:22 +0000</pubDate>
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					<description><![CDATA[Some of the simple techniques useful for students like me. Overnight 5ml subculture at 37 ℃ for 18hr. Inoculate 100µl subculture into 100 ml LB broth. Culture at 37 ℃, 2½ hr. Centrifuge at 4000 rpm, 10mins at 4°C (50ml +50ml). Re-suspend cell pellet in 5ml 0.1 M cacl2 for each tube. Keep on ice [&#8230;]]]></description>
		
		
		
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