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joke
I appreciate the effort, but next time remember we work on the squid giant axon, not the giant squid axon.
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CP cell preparation: 100 ml culture
Some of the simple techniques useful for students like me. Overnight 5ml subculture at 37 ℃ for 18hr. Inoculate 100µl subculture into 100 ml LB broth. Culture at 37 ℃, 2½ hr. Centrifuge at 4000 rpm, 10mins at 4°C (50ml +50ml). Re-suspend cell pellet in 5ml 0.1 M cacl2 for each tube. Keep on ice
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Scientists crack secrets of Mona Lisa
PARIS — The enigmatic smile remains a mystery, but French scientists say they have cracked a few secrets of the “Mona Lisa.” French researchers studied seven of the Louvre Museum's Leonardo da Vinci paintings, including the “Mona Lisa,” to analyze the master's use of successive ultrathin layers of paint and glaze — a technique that
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Trip to East Sea
Trip to East Sea (2008-6-4) Time flows like a river, never to be returned, who knows what life has stored for us. Trip to east-sea as I can describe; journey of eternity, felt like pursuit of happiness longed was fulfilled. I always wished to walk along sea beaches by bare foots. This dream of mine
